A Syringe-vacuum Driven Microfluidic Chip Integrated with Beads-based Elisa for Early Detection of Bladder Cancer
نویسندگان
چکیده
This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer biomarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beadsbased ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity( 99.2%) than conventional ELISA in plate.
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